Part:BBa_K4375007

Codon optimalised INPNC for Surface Display

INPNC codes for N- and C- terminal domain of Ice Nucleation Protein (INP) from Pseudomonas syringae, and it can be used for displaying proteins on bacteria's outer membrane.

Usage and Biology

The INP (Ice Nucleation Protein) is a membrane-bound protein from bacteria, like Pseudomonas and Erwinia, and can be used as an anchor motif for cell surface display. INPNC contains the N -and C-terminal domains of this protein in a truncated form. This is the codon optimized version of BBa_K811003 for expression in E. Coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 72
  Illegal NgoMIV site found at 405
  Illegal AgeI site found at 823
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

Wu, P.-H.; Giridhar, R.; Wu, W.-T. Surface Display of Transglucosidase onEscherichia Coli by Using the Ice Nucleation Protein ofXanthomonas Campestris and Its Application in Glucosylation of Hydroquinone. Biotechnology and Bioengineering 2006, 95 (6), 1138–1147. https://doi.org/10.1002/bit.21076.

https://2012.igem.org/Team:Penn/Team