RBS

Part:BBa_K4373005

Designed by: Mandy Hobusch   Group: iGEM22_Lund   (2022-09-24)


5'UTR including RBS for Bacillus subtilis

This part contains the 5'untranslated region of the Bacillus subtilis citB gene described by Fouet et al. We introduced here a strong consensus ribosome binding site (RBS) for Bacillus subtilis (BBa_K090505).

The part is used to initiate gene expression of glyphosate degrading proteins through ribosome binding.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The original RBS in the 5'UTR region from the citB gene was replaced with the strong consensus RBS (BBa_K090505). This enables the binding of Bacillus specific ribosomes and translation of downstream target genes is initiated.



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