DNA

Part:BBa_K4373003

Designed by: Mandy Hobusch, Leon Eisentraut   Group: iGEM22_Lund   (2022-09-24)


C-P-Lyase encoding sequence

Two pathways for the degradation of glyphosate have been described - the sacrosine pathway and the aminomethylphosphonic acid (AMPA) pathway. In the former reaction, an enzyme called C-P-Lyase catalyses the cleavage of C-P bonds from glyphosate forming sarcosine and organophosphate [1]. Many bacteria and fungi found in enviromental samples such as soil have been reported to perform this form of glyphosate degradation pathway [2]. For our project, the multimeric enzyme domain, so called CP-lyase was isolated from NCBI gene bank [3].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal PstI site found at 1654
    Illegal PstI site found at 3061
    Illegal PstI site found at 3360
    Illegal PstI site found at 3833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal PstI site found at 1654
    Illegal PstI site found at 3061
    Illegal PstI site found at 3360
    Illegal PstI site found at 3833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal BglII site found at 837
    Illegal BglII site found at 1605
    Illegal BamHI site found at 1461
    Illegal XhoI site found at 1056
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal PstI site found at 1654
    Illegal PstI site found at 3061
    Illegal PstI site found at 3360
    Illegal PstI site found at 3833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal PstI site found at 1654
    Illegal PstI site found at 3061
    Illegal PstI site found at 3360
    Illegal PstI site found at 3833
    Illegal NgoMIV site found at 2143
    Illegal NgoMIV site found at 3018
    Illegal NgoMIV site found at 3458
    Illegal AgeI site found at 662
    Illegal AgeI site found at 1121
    Illegal AgeI site found at 1497
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biology
protein