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Part:BBa_K4373002
Glyphosate dehydrogenase goxA
Two pathways for the degradation of glyphosate have been described - the sacrosine pathway and the aminomethylphosphonic acid (AMPA) pathway. In the ladder reaction, an enzyme called glyphosate dehydrogenase catalyses the cleavage of C-N bonds from glyphosate forming AMPA and glyoxylic acid [1]. Many bacteria found in enviromental samples such as soil have been reported to degrade glyphosate [2]. For our project, goxA, a gene encoding for glyphosate-degrading enzyme isolated from uncultured bacterium found in environmental samples has been utilized [3]. It was optimized for codon usage in Bacillus subtilis, our expression host. The FAD-dependent enzyme was produced intracellular and should be able to break down extracelluar glyphosate which is uptaken by the cell. This part has been described to have kinetic values of:
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 655
Illegal EcoRI site found at 943 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 300
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