Part:BBa_K4373002

Glyphosate dehydrogenase goxA

Two pathways for the degradation of glyphosate have been described - the sacrosine pathway and the aminomethylphosphonic acid (AMPA) pathway. In the ladder reaction, an enzyme called glyphosate dehydrogenase catalyses the cleavage of C-N bonds from glyphosate forming AMPA and glyoxylic acid [1]. Many bacteria found in enviromental samples such as soil have been reported to degrade glyphosate [2]. For our project, goxA, a gene encoding for glyphosate-degrading enzyme isolated from uncultured bacterium found in environmental samples has been utilized [3]. It was optimized for codon usage in Bacillus subtilis, our expression host. The FAD-dependent enzyme was produced intracellular and should be able to break down extracelluar glyphosate which is uptaken by the cell. This part has been described to have kinetic values of:

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 655
  Illegal EcoRI site found at 943
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 655
  Illegal EcoRI site found at 943
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 655
  Illegal EcoRI site found at 943
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 655
  Illegal EcoRI site found at 943
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 655
  Illegal EcoRI site found at 943
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 300