Part:BBa_K4373001
Glyphosate dehydrogenase goxB
Two pathways for the degradation of glyphosate have been described - the sacrosine pathway and the aminomethylphosphonic acid (AMPA) pathway. In the ladder reaction, an enzyme called glyphosate dehydrogenase catalyses the cleavage of C-N bonds from glyphosate forming AMPA and glyoxylic acid [1]. Many bacteria found in enviromental samples such as soil have been reported to degrade glyphosate [2] For our project, goxB, a gene encoding for glyphosate-degrading enzyme isolated from uncultured bacterium found in environmental samples has been utilized [3]. It was optimized for codon usage in Bacillus subtilis, our expression host. The FAD-dependent enzyme was produced intracellular and should be able to break down extracelluar glyphosate which is uptaken by the cell.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 760
- 1000COMPATIBLE WITH RFC[1000]
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