DNA

Part:BBa_K4373001

Designed by: Mandy Hobusch   Group: iGEM22_Lund   (2022-09-24)


Glyphosate dehydrogenase goxB

Two pathways for the degradation of glyphosate have been described - the sacrosine pathway and the aminomethylphosphonic acid (AMPA) pathway. In the ladder reaction, an enzyme called glyphosate dehydrogenase catalyses the cleavage of C-N bonds from glyphosate forming AMPA and glyoxylic acid [1]. Many bacteria found in enviromental samples such as soil have been reported to degrade glyphosate [2] For our project, goxB, a gene encoding for glyphosate-degrading enzyme isolated from uncultured bacterium found in environmental samples has been utilized [3]. It was optimized for codon usage in Bacillus subtilis, our expression host. The FAD-dependent enzyme was produced intracellular and should be able to break down extracelluar glyphosate which is uptaken by the cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 760
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
biology
protein