Regulatory

Part:BBa_K4370006

Designed by: Stéphanie Bury-Moné   Group: iGEM22_GO_Paris-Saclay   (2022-08-18)


KasOP*_promoter_STREPTO

The kasOP* promoter has been previously reported as one of the strongest promoter for an expression in Streptomyces (Bai et al., PNAS, 2015 ; Wang et al., Appl Environ Microbiol, 2013).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Chaoxian Bai , Yang Zhang , Xuejin Zhao, Yiling Hu, Sihai Xiang, Jin Miao, Chunbo Lou, Lixin Zhang “Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces”, Proc Natl Acad Sci U S A , 2015 Sep 29;112(39):12181-6. https://www.pnas.org/doi/10.1073/pnas.1511027112

Weishan Wang, Xiao Li, Juan Wang, Sihai Xiang, Xiaozhou Feng, Keqian Yang, “An engineered strong promoter for Streptomycetes”, Appl Environ Microbiol., 2013 Jul;79(14):4484-92. https://doi.org/10.1128/AEM.00985-13

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