DNA

Part:BBa_K4369005:Design

Designed by: Alexa Grebel   Group: iGEM22_Duesseldorf   (2022-10-11)


Cellulose synthase acsCD - Improvment of part BBa_K1321335


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1837
    Illegal BglII site found at 4399
    Illegal BamHI site found at 1906
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 778
    Illegal AgeI site found at 1334
    Illegal AgeI site found at 1913
    Illegal AgeI site found at 2123
    Illegal AgeI site found at 2975
    Illegal AgeI site found at 3293
    Illegal AgeI site found at 3638
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We improved part BBa_K1321335 with adding a His-Tag at the end to detect it with affinity protein purification when added to part BBa_K4369001 - Complete K. xylinus cellulose operon (adjusted for E. coli).


Source

BBa_K1321335

References