DNA

Part:BBa_K4369004:Design

Designed by: Alexa Grebel   Group: iGEM22_Duesseldorf   (2022-10-11)


Cellulose synthase acsAB - Improvment of part BBa_K1321334


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1831
    Illegal BglII site found at 2545
    Illegal BglII site found at 4468
    Illegal BamHI site found at 841
    Illegal BamHI site found at 3835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1817
    Illegal AgeI site found at 1894
    Illegal AgeI site found at 2450
    Illegal AgeI site found at 3251
    Illegal AgeI site found at 3343
    Illegal AgeI site found at 3449
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We improved part BBa_K1321334 with adding a His-Tag at the end to detect it with affinity protein purification when added to part BBa_K4369001 - Complete K. xylinus cellulose operon (adjusted for E. coli).


Source

BBa_K1321334

References