Composite
Part:BBa_K4362082
Designed by: Yun Wang Group: iGEM22_Tianjin (2022-09-11)
plasmid tightly regulated copy-control
This part consists of ParA-ParB-ParC plasmid partition system and oriS/oriV Copy-Control system. ParA-ParB-ParC plasmid partition system ensures the proper distribution of newly-replicated plasmids to daughter cells during cell division, when these proteins mutually impact. OriS/oriV Copy-Control system controls a single copy of the plasmid and implements a strictly controllable multicopy.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 729
Illegal SpeI site found at 3869
Illegal PstI site found at 1474
Illegal PstI site found at 2702 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 3869
Illegal PstI site found at 1474
Illegal PstI site found at 2702 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 683
Illegal BglII site found at 2353 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 729
Illegal SpeI site found at 3869
Illegal PstI site found at 1474
Illegal PstI site found at 2702 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 729
Illegal SpeI site found at 3869
Illegal PstI site found at 1474
Illegal PstI site found at 2702
Illegal NgoMIV site found at 89
Illegal NgoMIV site found at 96
Illegal NgoMIV site found at 220
Illegal NgoMIV site found at 1156
Illegal AgeI site found at 1280
Illegal AgeI site found at 2197
Illegal AgeI site found at 3097 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3951
Illegal SapI site found at 2946
[edit]
Categories
Parameters
None |