Composite

Part:BBa_K4362082

Designed by: Yun Wang   Group: iGEM22_Tianjin   (2022-09-11)


plasmid tightly regulated copy-control

This part consists of ParA-ParB-ParC plasmid partition system and oriS/oriV Copy-Control system. ParA-ParB-ParC plasmid partition system ensures the proper distribution of newly-replicated plasmids to daughter cells during cell division, when these proteins mutually impact. OriS/oriV Copy-Control system controls a single copy of the plasmid and implements a strictly controllable multicopy.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 729
    Illegal SpeI site found at 3869
    Illegal PstI site found at 1474
    Illegal PstI site found at 2702
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3869
    Illegal PstI site found at 1474
    Illegal PstI site found at 2702
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 683
    Illegal BglII site found at 2353
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 729
    Illegal SpeI site found at 3869
    Illegal PstI site found at 1474
    Illegal PstI site found at 2702
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 729
    Illegal SpeI site found at 3869
    Illegal PstI site found at 1474
    Illegal PstI site found at 2702
    Illegal NgoMIV site found at 89
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 220
    Illegal NgoMIV site found at 1156
    Illegal AgeI site found at 1280
    Illegal AgeI site found at 2197
    Illegal AgeI site found at 3097
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3951
    Illegal SapI site found at 2946


[edit]
Categories
Parameters
None