Terminator

Part:BBa_K4362064

Designed by: Yun Wang   Group: iGEM22_Tianjin   (2022-09-08)


Terminator.png

Synthetic terminator - 1

  • The following three elements were previously shown to be required for 3'-end formation of mRNA in the Saccharomyces cerevisiae: 3’-end formation of yeast mRNAs involves signals having the following three degenerate elements: (i) the efficiency element, which functions by enhancing the efficiency of positioning elements; (ii) the positioning element, which positions the poly(A) site; and (iii) the actual site of polyadenylation.[1]

The sequences between these sequences can be shortened by us, and reducing the homology of these sequences can reduce the repeatability. The filling of sequences is random, based on the research of Murray MacPherson et al [2].


Reference [1]. Guo, Z. J., and Sherman, F. (1996) Signals sufficient for 3′-end formation of yeast mRNA. Mol. Cell. Biol. 16, 2772−2776. [2]. MacPherson, M.; Saka, Y. Short Synthetic Terminators for Assembly of Transcription Units in Vitro and Stable Chromosomal Integration in Yeast S. cerevisiae. ACS Synth Biol 2017, 6 (1), 130-138.


Secondary Structure

Mfold-K4362064-1.png

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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