Part:BBa_K4362021

Ode To Joy-hyg

This is the plasmid that carried Huanlesun's sequence, and also, there is a HphNT1 gene on it to select in Saccharomyces cerevisiae

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 774
  Illegal XbaI site found at 3557
  Illegal SpeI site found at 3849
  Illegal PstI site found at 864
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 774
  Illegal SpeI site found at 3849
  Illegal PstI site found at 864
  Illegal NotI site found at 2534
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 774
  Illegal BamHI site found at 2561
  Illegal BamHI site found at 3839
  Illegal XhoI site found at 1558
  Illegal XhoI site found at 2510
  Illegal XhoI site found at 3256
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 774
  Illegal XbaI site found at 3557
  Illegal SpeI site found at 3849
  Illegal PstI site found at 864
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 774
  Illegal XbaI site found at 3557
  Illegal SpeI site found at 3849
  Illegal PstI site found at 864
  Illegal NgoMIV site found at 2167
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2787
  Illegal BsaI site found at 3315
  Illegal BsaI site found at 3583
  Illegal BsaI.rc site found at 3298
  Illegal BsaI.rc site found at 5227
  Illegal SapI site found at 4144
  Illegal SapI site found at 6248