Part:BBa_K4358014

Xylose operon with pqqC pqqD pqqE

Gene expression with xylose operon can be induced by the addition of xylose. When the inducer, xylose, is presented, xylose would bind to the xylose regulator protein, XylR, and change its structure. Hence, xylR wouldn’t bind to the operator(XylR-binding site) to inhibit the downstream gene of the xylR-binding site.

So we design the plasmid with Pxyl, Xylr and XylR-binding site for the pqqC, pqqD and pqqE Then we transformed pqqC, pqqD and pqqE in the downstream of the xylR-binding site in addition to controlling the expression of pqqC, pqqD and pqqE. If PqqC, PqqD and PqqE were required to be expressed , the xylose was present in the culture surrounding; if not, there was no PqqC, PqqD and PqqE.

Figure1. It illustrated the mechanism of how xylose regulator protein interacts with xylose promoter and inhibits downstream gene’s performance. The XylR would bind to the xylose binding site until the structure changed because of xylose.

Figure 2. xylose operon with pqqC pqqD pqqE (BBa_K4358014)