Plasmid

Part:BBa_K4358013

Designed by: HUNG. CHIEN-CHUN   Group: iGEM22_NCHU_Taichung   (2022-10-05)

Multi-pqqA pET-28a

Due to BBa_K3830012 used in 2021 NCHU_Taichung and the information displayed in wiki and registry, we knew that PqqA is a precursor for pyrroloquinoline quinone, PQQ, biosynthesis. Thus, the expression of pqqA is vital to the magnification of PQQ production. For the purpose of increasing the production of PQQ, we tried to double the pqqA gene in our plasmid to magnify the expression of pqqA to enhance PQQ production.

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Figure 1. Multi-PQQA pET28-A (BBa_K4358013)



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Figure 2. Wound healing assay (Normal HaCat cell) The HaCat cell was used to mimic wounds and the growth of the cells was observed to simulate the wound-healing situation. There were three treatments, Natto extracts, pyrroloquinoline quinone (PQQ), and the mixture treatments conducted and showed better recovery than the control group after 24hr culturing.


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Figure 3. Wound healing assay (Diabetes induced HaCat cell) The diabetic-like cells were induced by the medium supplemented with 25 mM glucose and 10 nM insulin and were treated with Natto extracts, pyrroloquinoline quinone(PQQ), and the mixture composed of both. Natto extracts, pyrroloquinoline quinone(PQQ), and the mixture treatments showed better recovery than the control group after 24hr culturing.

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