Part:BBa_K4358000

Promoter

xylose-inducible promoter

Gene expression with xylose promoter can be induced by the addition of xylose. When xylose is present, xylose will act as an inducer and bind to the xylose regulator protein to change its structure. Hence, XylR won’t bind to the operator (XylR-binding site) to inhibit the downstream gene of the XylR-binding site.

We designed the plasmid with Pxyl, XylR and XylR-binding site. Then we transformed pqqC, pqqD and pqqE in the downstream of the XylR-binding site in order to control the expression of pqqC, pqqD and pqqE to let more resources be used for the biosynthesis of PqqA and PqqB.

Figure1. It illustrated the mechanism of how xylose regulator protein interacts with xylose promoter and inhibits downstream gene’s performance. The XylR binds to the xylose binding site until the structure changes because of xylose.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]