Part:BBa_K4357005

J23100-XeR-mCherry-pS1C3

In the direction for our gene expression there is a promotor (BBa_J23100) from Constitutive promoter family member which has the strongest strength among BBa series of promoters in the Anderson promoter library, RBS (BBa_B0034) which is the ribosome binding site, XeR gene which is our target gene, controlling the expression of inward H proton channel , HRV 3C site which is the linker between target gene and reporter gene, mCherry gene which is our fluorescent reporter gene, 6xhis which is our his tag, double terminator (BBa_B0015) which is the terminator, BioBrick suffix and his Oberon terminator. In the opposite direction, the cat promotor controls the CmR gene which is the selective marker, carrying chloramphenicol resistance., followed by Iambda t0 terminator. There is also a replication origin, pUC19-derived pMB1 (copy number of 100-300 per cell).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 3774
  Illegal suffix found in sequence at 1726
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3774
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal SpeI site found at 1727
  Illegal PstI site found at 1741
  Illegal NotI site found at 1545
  Illegal NotI site found at 1734
  Illegal NotI site found at 3780
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3774
  Illegal BamHI site found at 794
  Illegal XhoI site found at 749
  Illegal XhoI site found at 1554
  Illegal XhoI site found at 2758
  Illegal XhoI site found at 3650
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 3774
  Illegal suffix found in sequence at 1727
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 3774
  Illegal XbaI site found at 3789
  Illegal SpeI site found at 1727
  Illegal PstI site found at 1741
  
• 1000
  COMPATIBLE WITH RFC[1000]