Part:BBa_K4357001

Pasr-XeR-mCherry-pSB1C3

In the direction for our gene expression, there is an asr promotor (BBa_K1231000) which is the pH-responsive promoter native to E. coli, inducing transcription in acidic conditions (~pH 5.5), RBS (BBa_B0034) which is the ribosome binding site, XeR gene which is our target gene, controlling the expression of inward H proton channel, HRV 3C site which is the linker between the target gene and reporter gene, mCherry gene which is our fluorescent reporter gene, 6xhis which is our his tag, double terminator (BBa_B0015) which is the terminator, BioBrick suffix and his Oberon terminator. In the opposite direction, the cat promotor controls the CmR gene which is the selective marker, carrying chloramphenicol resistance., followed by Iambda t0 terminator. There is also a replication origin, pUC19-derived pMB1 (copy number of 100-300 per cell).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 3879
  Illegal suffix found in sequence at 1831
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3879
  Illegal SpeI site found at 1832
  Illegal PstI site found at 1846
  Illegal NotI site found at 1650
  Illegal NotI site found at 1839
  Illegal NotI site found at 3885
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3879
  Illegal BamHI site found at 899
  Illegal XhoI site found at 854
  Illegal XhoI site found at 1659
  Illegal XhoI site found at 2863
  Illegal XhoI site found at 3755
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 3879
  Illegal suffix found in sequence at 1832
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 3879
  Illegal XbaI site found at 3894
  Illegal SpeI site found at 1832
  Illegal PstI site found at 1846
  
• 1000
  COMPATIBLE WITH RFC[1000]