Composite

Part:BBa_K4354013

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-29)


CcaS/CcaR green-light regulating system in Bacillus subtilis

We create this BioBrick to improve the CcaR/CcaS green-light regulating system in Bacillus subtilis. Under the green light, Bacillus subtilis suicides itself because of gene NucA's expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1201
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2580
    Illegal PstI site found at 1201
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1211
    Illegal BglII site found at 1331
    Illegal XhoI site found at 4226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1201
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1201
    Illegal AgeI site found at 3244
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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