Plasmid

Part:BBa_K4354010

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-29)


plasmid pHY-P43

Plasmid pHY-P43 is an expression vector existing in Bacillus subtilis，it is used to express AMO,HAO,NAR,nirS and GFP to remove the total nitrogen in wasted water.It has Ampicillin and Tetracycline resistance.(5166bp)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 333
    Illegal PstI site found at 311
    Illegal PstI site found at 4126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 333
    Illegal PstI site found at 311
    Illegal PstI site found at 4126
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 333
    Illegal BglII site found at 307
    Illegal BamHI site found at 323
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 333
    Illegal PstI site found at 311
    Illegal PstI site found at 4126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 333
    Illegal PstI site found at 311
    Illegal PstI site found at 4126
    Illegal AgeI site found at 5005
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2883
    Illegal BsaI site found at 4300


[edit]
Categories
Parameters
None