Composite

Part:BBa_K4354009

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-28)


BioBrick used to express EL222

This BioBrick is composed of P43 strong promoter and EL222 photo-sensitive protein coding gene. We will clone it to vector pUC19,a high copy plasmid replicating in Bacillus subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 898
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 468
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None