Composite

Part:BBa_K4354008

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-28)


BioBrick used to express enzymes about total nitrogen removal and report gene GFP

Plasmid pHY-P43 is a expression vector from Bacillus subtilis. We choose it to express specific genes in our engineering Bacillus subtilis. It has ampicillin resistance and tetracycline resistance. It is a medium-low copy vector about 5166bp.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 986
    Illegal EcoRI site found at 4660
    Illegal PstI site found at 1851
    Illegal PstI site found at 3007
    Illegal PstI site found at 3013
    Illegal PstI site found at 3771
    Illegal PstI site found at 5066
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 986
    Illegal EcoRI site found at 4660
    Illegal NheI site found at 2987
    Illegal PstI site found at 1851
    Illegal PstI site found at 3007
    Illegal PstI site found at 3013
    Illegal PstI site found at 3771
    Illegal PstI site found at 5066
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 986
    Illegal EcoRI site found at 4660
    Illegal BglII site found at 2572
    Illegal BamHI site found at 1687
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 986
    Illegal EcoRI site found at 4660
    Illegal PstI site found at 1851
    Illegal PstI site found at 3007
    Illegal PstI site found at 3013
    Illegal PstI site found at 3771
    Illegal PstI site found at 5066
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 501
    Illegal EcoRI site found at 986
    Illegal EcoRI site found at 4660
    Illegal PstI site found at 1851
    Illegal PstI site found at 3007
    Illegal PstI site found at 3013
    Illegal PstI site found at 3771
    Illegal PstI site found at 5066
    Illegal NgoMIV site found at 4235
    Illegal NgoMIV site found at 4427
    Illegal AgeI site found at 4814
    Illegal AgeI site found at 5431
    Illegal AgeI site found at 5873
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 884
    Illegal BsaI.rc site found at 1696
    Illegal BsaI.rc site found at 5183
    Illegal BsaI.rc site found at 5462
    Illegal BsaI.rc site found at 6567
    Illegal SapI.rc site found at 1839


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