Coding
nirS

Part:BBa_K4354007

Designed by: Java Yao   Group: iGEM22_GXU-China   (2022-07-22)


nirS nitrite reductase[Pseudomonas stutzeri]

nirS is a gene coding an enzyme that has the ability to turn nitrite ion to nitric oxide.It is very important for nitrogen removal in wasted water because it can transfer the ion to the gas and make it out of water.We use it to build an engineering Bacillus subtilis to purify the water.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 537
    Illegal PstI site found at 943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 537
    Illegal PstI site found at 943
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 537
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 537
    Illegal PstI site found at 943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 537
    Illegal PstI site found at 943
    Illegal NgoMIV site found at 112
    Illegal NgoMIV site found at 304
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 1750
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1060
    Illegal BsaI.rc site found at 1339


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Parameters
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