Regulatory

Part:BBa_K4351013

Designed by: Laura Keffer-Wilkes   Group: iGEM22_Lethbridge_HS   (2022-10-07)


Theophylline RNA Aptamer

The theophylline biosensor was to act as our positive control in testing our glucose biosensors as it has been well characterized and binds theophylline tightly in vitro with a sub micromolar affinity. This part is different than the currently available iGEM parts BBa_J89000 and BBa_K784005.

Fluorescence experiments were completed to measure glucose binding by the aptamer by combining the control biosensors with RNA Mango part (BBa_K4351010).

For more information on how this part was used visit:https://parts.igem.org/Part:BBa_K4351021

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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