Regulatory
Part:BBa_K4351013
Designed by: Laura Keffer-Wilkes Group: iGEM22_Lethbridge_HS (2022-10-07)
Theophylline RNA Aptamer
The theophylline biosensor was to act as our positive control in testing our glucose biosensors as it has been well characterized and binds theophylline tightly in vitro with a sub micromolar affinity. This part is different than the currently available iGEM parts BBa_J89000 and BBa_K784005.
Fluorescence experiments were completed to measure glucose binding by the aptamer by combining the control biosensors with RNA Mango part (BBa_K4351010).
For more information on how this part was used visit:https://parts.igem.org/Part:BBa_K4351021
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |