Part:BBa_K4351007
VEEV NSP3
The 5′ and 3′ Conserved Sequence Elements, subgenomic promoter, and non-structural proteins were all acquired from a VEEV genome. Specifically, the VEEV replicon vector YFV-C1 submitted by Shustov, A.V., and Frolov, I.V. to the NIH National Library of Medicine GenBank (GenBank: DQ322637.1). NSP1-4 are translated as one large protein which breaks into several individual proteins through autolytic activity (8). Together, they function as two different RNA-dependent RNA polymerases, first making complementary negative sense RNA, then more positive sense RNA that the ribosome can use as templates for translation (9). The subgenomic promoter will encourage the RNA-dependent RNA polymerase to produce an additional mRNA containing only the GOI (9).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 426
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1297
Illegal PstI site found at 426 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1461
Illegal XhoI site found at 1522
Illegal XhoI site found at 1563 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 426
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 426
Illegal AgeI site found at 1002
Illegal AgeI site found at 1371 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1560
Illegal BsaI site found at 1578
Illegal BsaI.rc site found at 1087
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