Composite
Part:BBa_K4350832
Designed by: Ryan Riopel Group: iGEM22_UBC-Okanagan (2022-10-12)
Fungal Bioluminescence Multi-Gene Construct
This multi-gene construct is designed for the expression of the fungal bioluminescence pathway in Synechocystis sp. PCC 6803. The genes included encode fungal luciferase, CPH, H3H, NpgA, and HispS codon-optimized for expression in Synechocystis sp. PCC 6803. This plasmid was assembled by UBC-Okanagan iGEM 2022 using golden gate cloning.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 4610
Illegal SpeI site found at 5741
Illegal PstI site found at 3522
Illegal PstI site found at 5451
Illegal PstI site found at 7720
Illegal PstI site found at 8473
Illegal PstI site found at 8689 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1907
Illegal SpeI site found at 5741
Illegal PstI site found at 3522
Illegal PstI site found at 5451
Illegal PstI site found at 7720
Illegal PstI site found at 8473
Illegal PstI site found at 8689 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6491
Illegal BglII site found at 9464
Illegal BamHI site found at 3631
Illegal BamHI site found at 5927
Illegal BamHI site found at 6636
Illegal XhoI site found at 448
Illegal XhoI site found at 3285
Illegal XhoI site found at 4122 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 4610
Illegal SpeI site found at 5741
Illegal PstI site found at 3522
Illegal PstI site found at 5451
Illegal PstI site found at 7720
Illegal PstI site found at 8473
Illegal PstI site found at 8689 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 4610
Illegal SpeI site found at 5741
Illegal PstI site found at 3522
Illegal PstI site found at 5451
Illegal PstI site found at 7720
Illegal PstI site found at 8473
Illegal PstI site found at 8689
Illegal NgoMIV site found at 7644
Illegal AgeI site found at 5842
Illegal AgeI site found at 8299
Illegal AgeI site found at 8982 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 9732
Illegal BsaI site found at 9910
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Categories
Parameters
None |