Composite

Part:BBa_K4350832

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-12)


Fungal Bioluminescence Multi-Gene Construct

This multi-gene construct is designed for the expression of the fungal bioluminescence pathway in Synechocystis sp. PCC 6803. The genes included encode fungal luciferase, CPH, H3H, NpgA, and HispS codon-optimized for expression in Synechocystis sp. PCC 6803. This plasmid was assembled by UBC-Okanagan iGEM 2022 using golden gate cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4610
    Illegal SpeI site found at 5741
    Illegal PstI site found at 3522
    Illegal PstI site found at 5451
    Illegal PstI site found at 7720
    Illegal PstI site found at 8473
    Illegal PstI site found at 8689
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1907
    Illegal SpeI site found at 5741
    Illegal PstI site found at 3522
    Illegal PstI site found at 5451
    Illegal PstI site found at 7720
    Illegal PstI site found at 8473
    Illegal PstI site found at 8689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6491
    Illegal BglII site found at 9464
    Illegal BamHI site found at 3631
    Illegal BamHI site found at 5927
    Illegal BamHI site found at 6636
    Illegal XhoI site found at 448
    Illegal XhoI site found at 3285
    Illegal XhoI site found at 4122
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4610
    Illegal SpeI site found at 5741
    Illegal PstI site found at 3522
    Illegal PstI site found at 5451
    Illegal PstI site found at 7720
    Illegal PstI site found at 8473
    Illegal PstI site found at 8689
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4610
    Illegal SpeI site found at 5741
    Illegal PstI site found at 3522
    Illegal PstI site found at 5451
    Illegal PstI site found at 7720
    Illegal PstI site found at 8473
    Illegal PstI site found at 8689
    Illegal NgoMIV site found at 7644
    Illegal AgeI site found at 5842
    Illegal AgeI site found at 8299
    Illegal AgeI site found at 8982
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9732
    Illegal BsaI site found at 9910


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