Composite

Part:BBa_K4350828

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


nnHispS Transcriptional Unit

Transcriptional unit for the expression of N. nambi hispidin synthase. Assembled as a level 1 MoClo part compatible for assembly into a level 2 multi-gene construct. Composed of the PlacIQ synthetic constitutive promoter based on the PlacI promoter from E. coli, the nnHispS coding sequence, and TrrnB terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 100
    Illegal SpeI site found at 1231
    Illegal PstI site found at 941
    Illegal PstI site found at 3210
    Illegal PstI site found at 3963
    Illegal PstI site found at 4179
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1231
    Illegal PstI site found at 941
    Illegal PstI site found at 3210
    Illegal PstI site found at 3963
    Illegal PstI site found at 4179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1981
    Illegal BglII site found at 4954
    Illegal BamHI site found at 1417
    Illegal BamHI site found at 2126
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 100
    Illegal SpeI site found at 1231
    Illegal PstI site found at 941
    Illegal PstI site found at 3210
    Illegal PstI site found at 3963
    Illegal PstI site found at 4179
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 100
    Illegal SpeI site found at 1231
    Illegal PstI site found at 941
    Illegal PstI site found at 3210
    Illegal PstI site found at 3963
    Illegal PstI site found at 4179
    Illegal NgoMIV site found at 3134
    Illegal AgeI site found at 1332
    Illegal AgeI site found at 3789
    Illegal AgeI site found at 4472
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5222
    Illegal BsaI site found at 5400


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Categories
Parameters
None