Composite
Part:BBa_K4350828
Designed by: Ryan Riopel Group: iGEM22_UBC-Okanagan (2022-10-09)
nnHispS Transcriptional Unit
Transcriptional unit for the expression of N. nambi hispidin synthase. Assembled as a level 1 MoClo part compatible for assembly into a level 2 multi-gene construct. Composed of the PlacIQ synthetic constitutive promoter based on the PlacI promoter from E. coli, the nnHispS coding sequence, and TrrnB terminator.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 100
Illegal SpeI site found at 1231
Illegal PstI site found at 941
Illegal PstI site found at 3210
Illegal PstI site found at 3963
Illegal PstI site found at 4179 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1231
Illegal PstI site found at 941
Illegal PstI site found at 3210
Illegal PstI site found at 3963
Illegal PstI site found at 4179 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1981
Illegal BglII site found at 4954
Illegal BamHI site found at 1417
Illegal BamHI site found at 2126 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 100
Illegal SpeI site found at 1231
Illegal PstI site found at 941
Illegal PstI site found at 3210
Illegal PstI site found at 3963
Illegal PstI site found at 4179 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 100
Illegal SpeI site found at 1231
Illegal PstI site found at 941
Illegal PstI site found at 3210
Illegal PstI site found at 3963
Illegal PstI site found at 4179
Illegal NgoMIV site found at 3134
Illegal AgeI site found at 1332
Illegal AgeI site found at 3789
Illegal AgeI site found at 4472 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5222
Illegal BsaI site found at 5400
[edit]
Categories
Parameters
None |