Composite

Part:BBa_K4350827

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


NpgA Transcriptional Unit

Transcriptional unit for the expression of A. nidulans NpgA. Assembled as a level 1 MoClo part compatible for assembly into a level 2 multi-gene construct. Composed of the PlacIQ synthetic constitutive promoter based on the PLacI promoter from E. coli, the NpgA coding sequence, and TpheA-1 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 146
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 255
    Illegal XhoI site found at 746
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 146
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None