Composite

Part:BBa_K4350822

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


nnLuz Transcriptional Unit with PBAD Promoter

Transcriptional unit for the expression of N. nambi fungal luciferase. Assembled as a level 1 MoClo part compatible for assembly into a level 2 multi-gene construct. Composed of the PBAD arabinose-inducible promoter from E. coli, the nnLuz coding sequence, and Tcpc terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1621
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None