Part:BBa_K4350820

N. nambi Fungal Luciferase V49A d33

Coding sequence for the fungal luciferase enzyme from Neonothopanus nambi with a V49A mutation and a deletion of 33 N-terminal amino acids. Beregovaya et al. (2021) found that a V49A mutation in the luciferase gene results in a significant increase in the luminescence of engineered P. pastoris cultures. Additionally, they determined that deletions of up to 37 N-terminal amino acids does not result in a loss of enzyme function, as the N-terminal possesses a transmembrane domain.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

• Beregovaya, K. A., Myshkina, N. M., Chepurnykh, T. V., Kotlobay, A. A., Purtov, K. V., Petushkov, V. N., Rodionova, N. S., & Yampolsky, I. V. (2021). Rational design and mutagenesis of fungal luciferase from Neonothopanus Nambi. Doklady Biochemistry and Biophysics, 496(1), 14–17. https://doi.org/10.1134/s1607672921010026