Coding
Part:BBa_K4350819
Designed by: Ryan Riopel Group: iGEM22_UBC-Okanagan (2022-10-09)
N. nambi Fungal Luciferase V49A
Coding sequence for the fungal luciferase enzyme from Neonothopanus nambi with a V49A mutation. Beregovaya et al. (2021) found that a V49A mutation in the luciferase gene results in a significant increase in the luminescence of engineered P. pastoris cultures.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 392
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- Beregovaya, K. A., Myshkina, N. M., Chepurnykh, T. V., Kotlobay, A. A., Purtov, K. V., Petushkov, V. N., Rodionova, N. S., & Yampolsky, I. V. (2021). Rational design and mutagenesis of fungal luciferase from Neonothopanus Nambi. Doklady Biochemistry and Biophysics, 496(1), 14–17. https://doi.org/10.1134/s1607672921010026
[edit]
Categories
Parameters
None |