Part:BBa_K4350817

A. nidulans 4′-Phosphopantetheinyl Transferase (NpgA)

The 4’-phosphopantetheinyl transferase enzyme of A. nidulans (NpgA) is responsible for the post-translational modification of hispidin synthase (HispS) (Kotlobay et al., 2018). Through the addition of a phosphopantetheinyl moiety, NpgA converts HispS into its holo-form. Heterologous expression of HispS also requires expression of a phosphopantetheinyl transferase enzyme for proper functioning (Kotlobay et al., 2018).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 109
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 109
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 218
  Illegal XhoI site found at 709
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 109
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 109
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

• Kotlobay, A. A., Sarkisyan, K. S., Mokrushina, Y. A., Marcet-Houben, M., Serebrovskaya, E. O., Markina, N. M., Gonzalez Somermeyer, L., Gorokhovatsky, A. Y., Vvedensky, A., Purtov, K. V., Petushkov, V. N., Rodionova, N. S., Chepurnyh, T. V., Fakhranurova, L. I., Guglya, E. B., Ziganshin, R., Tsarkova, A. S., Kaskova, Z. M., Shender, V., … Yampolsky, I. V. (2018). Genetically encodable bioluminescent system from fungi. Proceedings of the National Academy of Sciences, 115(50), 12728–12732. https://doi.org/10.1073/pnas.1803615115