Part:BBa_K4350816

N. nambi Hispidin 3-Hydroxylase (nnH3H)

Coding sequence for the enzyme that catalyzes the formation of fungal luciferin (3-hydroxyhispidin) from hispidin in the fungal bioluminescence pathway of N. nambi.

nnH3H is an NAD(P)H-dependent monooxygenase that catalyzes the formation of 3-hydroxyhispidin from hispidin (Kotlobay et al., 2018). It is the second enzyme in the fungal bioluminescence pathway, responsible for the formation of fungal luciferin, the substrate of the light-emitting reaction in bioluminescent fungi (Kotlobay et al., 2018). This enzyme connects the fungal bioluminescence pathway with a common fungal metabolite, hispidin (Kotlobay et al., 2018).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1234
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

• Kotlobay, A. A., Sarkisyan, K. S., Mokrushina, Y. A., Marcet-Houben, M., Serebrovskaya, E. O., Markina, N. M., Gonzalez Somermeyer, L., Gorokhovatsky, A. Y., Vvedensky, A., Purtov, K. V., Petushkov, V. N., Rodionova, N. S., Chepurnyh, T. V., Fakhranurova, L. I., Guglya, E. B., Ziganshin, R., Tsarkova, A. S., Kaskova, Z. M., Shender, V., … Yampolsky, I. V. (2018). Genetically encodable bioluminescent system from fungi. Proceedings of the National Academy of Sciences, 115(50), 12728–12732. https://doi.org/10.1073/pnas.1803615115
• Vasudevan, R., Gale, G. A. R., Schiavon, A. A., Puzorjov, A., Malin, J., Gillespie, M. D., Vavitsas, K., Zulkower, V., Wang, B., Howe, C. J., Lea-Smith, D. J., & McCormick, A. J. (2019). CyanoGate: A modular cloning suite for engineering cyanobacteria based on the plant MoClo syntax. Plant Physiology, 180(1), 39–55. https://doi.org/10.1104/pp.18.01401