Part:BBa_K4350815

N. nambi Caffeylpyruvate Hydrolase (nnCPH)

Caffeylpyruvate hydrolase (CPH) is an enzyme responsible for catalyzing the final step of the fungal bioluminescence pathway (Kotlobay et al., 2018). CPH is a member of the fumarylacetoacetate hydrolase family of enzymes, and catalyzes the creation of caffeic acid from caffeylpyruvic acid, releasing pyruvic acid in the process (Kotlobay et al., 2018). CPH recycles the product of the light-emitting reaction in the fungal bioluminescence pathway into caffeic acid, the precursor of fungal luciferin, thus completing the caffeic acid cycle (Kotlobay et al., 2018).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 901
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

• Kotlobay, A. A., Sarkisyan, K. S., Mokrushina, Y. A., Marcet-Houben, M., Serebrovskaya, E. O., Markina, N. M., Gonzalez Somermeyer, L., Gorokhovatsky, A. Y., Vvedensky, A., Purtov, K. V., Petushkov, V. N., Rodionova, N. S., Chepurnyh, T. V., Fakhranurova, L. I., Guglya, E. B., Ziganshin, R., Tsarkova, A. S., Kaskova, Z. M., Shender, V., … Yampolsky, I. V. (2018). Genetically encodable bioluminescent system from fungi. Proceedings of the National Academy of Sciences, 115(50), 12728–12732. https://doi.org/10.1073/pnas.1803615115