Composite

Part:BBa_K4349026

Designed by: Paulo Aguiar Muniz   Group: iGEM22_UFMG_UFV_Brazil   (2022-10-12)


Repressible promoter encoding specific Lactobacillus endotoxin

This part is composed of a promoter repressed by SrpR (BBa_K1725020). This promoter codes for a lactobacillus endotoxin called endolysin (BBa_K4133012)

Synthetic lactobacillus endotoxin gene.jpeg

This image summarizes the components and functioning of the parts created to compose the second and last stage of the kill switch circuit for the cell death of lactobacillus. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 20
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]


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