Composite

Part:BBa_K4349022

Designed by: Paulo Aguiar Muniz   Group: iGEM22_UFMG_UFV_Brazil   (2022-10-12)


Regulation gene by cIts coding

This part is composed of a constitutive promoter (BBa_J23107) from the J23100 to J23119 family, an RBS region (BBa_B0034) and a temperature-dependent repressor cIts coding region. It produces a temperature-sensitive cI repressor that loses binding efficiency with the cI promoter (BBa_R0051) between 35 and 42 degrees Celsius. This part was not characterized.

Temp Protein Gene UFMG UFV.jpeg

This image summarizes the components and functioning of the parts created to compose the first stage of the kill switch circuit for lactobacillus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None