Signalling

Part:BBa_K4349002

Designed by: Vanessa Pasa   Group: iGEM22_UFMG_UFV_Brazil   (2022-08-02)


Cwh signal peptide from Lactobacillus casei

Cwh is a signal peptide that promotes the secretion of proteins in Lactic acid bacteria.

Usage and Biology

The Cwh sequence was selected from the genome of Lactocabillus casei via bioinformatic analysis. Then, the secretion capacity of Cwh was tested with the reporter protein GFP in Lactobacillus casei RCEID02. It showed the highest efficiency among the putative signal peptides selected in the study. Cwh was also combined with the heterologous protein M2e:HBc and was able to successfully secrete the protein (Themsakul et al., 2016).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Themsakul, S., Suebwongsa, N., Mayo, B., Panya, M., & Lulitanond, V. (2016). Secretion of M2e:HBc fusion protein by Lactobacillus casei using Cwh signal peptide. FEMS Microbiology Letters, 363(19), fnw209. https://doi.org/10.1093/femsle/fnw209

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Categories
Parameters
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