Composite

Part:BBa_K4345023

Designed by: Luka Van den Berghe   Group: iGEM22_KU_Leuven   (2022-10-08)


5' UTR of rpoH with ccdA fused to sfGFP

Design of a thermoregulated translation of antitoxin ccdA. Fluorescent protein sfGFP was linked to the antitoxin with a rigid linker for visualisation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 767
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The figure below shows the secondary structure of the RNA thermometer.

RpoH Morita et al 1999.jpeg

Image obtained from Morita et al., 1999.


References

Morita, M. T., Tanaka, Y., Kodama, T. S., Kyogoku, Y., Yanagi, H., & Yura, T. (1999, March 15). Translational induction of heat shock transcription factor sigma 32: evidence for a built-in RNA thermosensor. Genes &Amp; Development, 13(6), 655–665. https://doi.org/10.1101/gad.13.6.655

[edit]
Categories
//biosafety/kill_switch
//cds/reporter/gfp
//regulation/constitutive
//rna/riboswitch
Parameters
biology
control
emission
excitation