Regulatory
Tacolaco

Part:BBa_K4344082

Designed by: Marcel Pott   Group: iGEM22_Heidelberg   (2022-09-30)


Tac promoter-LacO-Shine Dalgarno

We improved the iGEM Part BBa_K2172009 by adding E. coli specific Shine-Dalgarno Sequence to enhance Ribosome binding and therefore enhance protein expression in comparison to the annotated ribosome binding site. The spacer sequence betweeen the tac-promoter and the lac-operator is the the sequence with the highest acivity published in the paper Mulligan, M. E., Brosius, J., & McClure, W. R., 1985.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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