Primer

Part:BBa_K4344045

Designed by: Franziska Lam   Group: iGEM22_Heidelberg   (2022-09-29)


pRK5-Kozak-fwd.

Part of our primer set used for inserting Kozak-sequence together with following parts: BBa_K4344007 (EcoRI-HSV UL19), BBa_K4344018 (HindIII-HSV UL19-rev.), BBa_K4344045 (pRK5-Kozak-fwd.). EcoRI-HSV-UL19 primer was not used in the final project, but still works.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 27
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 27
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 27
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 27
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 27
  • 1000
    COMPATIBLE WITH RFC[1000]


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