Composite

Part:BBa_K4343152

Designed by: Zhe Zheng   Group: iGEM22_NNU-China   (2022-10-10)


nCBE

A cytosine base editor with TEF promoter-driven expression in which the Cas9 protein is nCas9. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1546
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4876
    Illegal BamHI site found at 3825
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 437
    Illegal BsaI site found at 5476
    Illegal BsaI.rc site found at 423
    Illegal BsaI.rc site found at 4557
    Illegal BsaI.rc site found at 5740


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Categories
Parameters
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