Coding

Part:BBa_K4343139

Designed by: Zheng Zhe   Group: iGEM22_NNU-China   (2022-10-09)


dCas9

dCas9 (dead Cas9) is a Cas9 protein that has lost its cleavage activity , containing two mutations (D10A and H840A).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 4121


[edit]
Categories
Parameters
None