Regulatory

Part:BBa_K4343125

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


GAP

Derived from Y. lipolytica, GAP promotor is a specific DNA sequence upstream of the 5' end of a structural gene that can be specifically recognized and bound by RNAP. Some researchers have characterized multiple promoters by reporters, among which the GAP promoter facilitates the expression.

Reference: Wong, L., Engel, J., Jin, E., Holdridge, B., & Xu, P. (2017). YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway engineering in Yarrowia lipolytica. Metabolic engineering communications, 5, 68-77.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 564


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