Coding

Part:BBa_K4343106

Designed by: Xiner Liu   Group: iGEM22_NNU-China   (2022-10-09)


PpElo9

PpElo9 encodes ∆-9 elongase from Pavlova pinguis which functions in the Δ-9 elongase/desaturase pathway and could convert C18:2 to C20:2.

Characterization

Heterologous expression of PpElo9 could enable engineered Y. lipolytica to produce C20:2 fatty acids that couldn’t be produced in wild-type Y. lipolytica.

Literature

Petrie, JR described the isolation of three additional Delta 9-elongases from the class Haptophyceae and demonstrate that the Delta 9-elongase group contains highly conserved regions, which differentiate them from other ELO-type elongases.

Reference

Petrie, J. R., Mackenzie, A. M., Shrestha, P., Liu, Q., Frampton, D. F., Robert, S. S., & Singh, S. P. (2010). ISOLATION OF THREE NOVEL LONG‐CHAIN POLYUNSATURATED FATTY ACID Δ9‐ELONGASES AND THE TRANSGENIC ASSEMBLY OF THE ENTIRE PAVLOVA SALINA DOCOSAHEXAENOIC ACID PATHWAY IN NICOTIANA BENTHAMIANA 1. Journal of Phycology, 46(5), 917-925.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 585
    Illegal BsaI.rc site found at 836


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Categories
Parameters
None