DNA

Part:BBa_K4343064

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-08)


SCP2-up

SCP2- up is 1.5kb upstream scp2 site of Y. lipoltiyca, which is part of the donor DNA, allowing for the homologous recombination to integrate various ∆-9 elongase and C16/18 elongase gene of different species with different strength of promoter, hence verifying their contributions to EPA production and further optimizing our engineered strain based on the results of the study.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 889
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 401
    Illegal BsaI.rc site found at 1540


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Categories
Parameters
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