DNA

Part:BBa_K4343061

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-08)


PEX10 down-V2

PEX10 down-V2 is 1kb downstream the gene PEX10 in the genome of Y. lipoltiyca, which is part of the donor DNA, allowing for the homologous recombination to knock out gene PEX10 to block oxidation pathway in Y. lipoltiyca, hence improving EPA production. This segment is ligated to the screening marker URA gene and PEX10 up-V2, which had been explained before.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 633
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1517


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Parameters
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