DNA

Part:BBa_K4343060

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-08)


PEX10 up-V2

PEX10 up-V2 is 1kb upstream the gene PEX10 in the genome of Y. lipoltiyca, which is part of the donor DNA, allowing for the homologous recombination to knock out gene PEX10 to block oxidation pathway in Y. lipoltiyca, hence improving EPA production. This segment is ligated to the screening marker URA gene and PEX10 down-V2, which would be explained as follows.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1136
    Illegal BglII site found at 1589
    Illegal BglII site found at 1819
    Illegal BamHI site found at 236
    Illegal BamHI site found at 392
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 570
    Illegal BsaI site found at 1251
    Illegal BsaI.rc site found at 2263


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