Composite

Part:BBa_K4336005

Designed by: YI TING LEE   Group: iGEM22_NTHU_Taiwan   (2022-10-07)


[Ranalexin] E [Ranalexin]' E [Lysostaphin]

We designed an antimicrobial peptide complex using the V8 protease cleavage site as a linker between our antimicrobial peptides. when encountering Staphylococcus aureus, linker between antimicrobial peptides will be cleaved by V8 protease and release antimicrobial peptides which can kill Staphylococcus aureus. However, AMPC will remain as an inactive form as long as the linker exists.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 70
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 70
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 70
    Illegal BamHI site found at 522
    Illegal XhoI site found at 877
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 70
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 70
    Illegal NgoMIV site found at 99
    Illegal NgoMIV site found at 547
    Illegal NgoMIV site found at 562
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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