Composite
Part:BBa_K4336005
Designed by: YI TING LEE Group: iGEM22_NTHU_Taiwan (2022-10-07)
[Ranalexin] E [Ranalexin]' E [Lysostaphin]
We designed an antimicrobial peptide complex using the V8 protease cleavage site as a linker between our antimicrobial peptides. when encountering Staphylococcus aureus, linker between antimicrobial peptides will be cleaved by V8 protease and release antimicrobial peptides which can kill Staphylococcus aureus. However, AMPC will remain as an inactive form as long as the linker exists.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 70
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 70
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 70
Illegal BamHI site found at 522
Illegal XhoI site found at 877 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 70
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 70
Illegal NgoMIV site found at 99
Illegal NgoMIV site found at 547
Illegal NgoMIV site found at 562 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |