Composite

Part:BBa_K4336003

Designed by: YI TING LEE   Group: iGEM22_NTHU_Taiwan   (2022-10-07)


E(LL37) E (hBD-3) E (LL37) E (hBD-3) E(LL37) E (hBD-3)

We designed an antimicrobial peptide complex using the V8 protease cleavage site as a linker between our antimicrobial peptides. when encountering Staphylococcus aureus, linker between antimicrobial peptides will be cleaved by V8 protease and release antimicrobial peptides which can kill Staphylococcus aureus. However, AMPC will remain as an inactive form as long as the linker exists.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 742
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 202
    Illegal SapI.rc site found at 454


[edit]
Categories
Parameters
None