Composite

Part:BBa_K4336000

Designed by: YI TING LEE   Group: iGEM22_NTHU_Taiwan   (2022-10-07)


[LL37]-linker-[LL37]’-linker-[Lysostaphin]

We designed an antimicrobial peptide complex using the V8 protease cleavage site as a linker between our antimicrobial peptides. when encountering Staphylococcus aureus, linker between antimicrobial peptides will be cleaved by V8 protease and release antimicrobial peptides which can kill Staphylococcus aureus. However, AMPC will remain as an inactive form as long as the linker exists.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 624
    Illegal XhoI site found at 979
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 664
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None