Part:BBa_K4324101
Xylitol Dehydrogenase from S. cerevisiae
This part is the CDS of the XYL2 gene from S. cerevisiae that induces xylitol dehydrogenase, and has been codon-optimised for expression in E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 606
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 263
Usage and Biology
Our project focused on the improvement of xylose utilisation in E. coli. One part of this process was to incorporate a yeast-derived XR-XDH pathway
Xylitol dehydrogenase (EC 1.1.1.9), an oxidoreductase, is an enzyme that serves as a catalyst for the conversion of xylitol into xylulose, and vice versa, according to the following chemical equation:
In S. cerevisiae yeast cells, xylitol dehydrogenase forms the second process in the XR-XDH pathway, as shown in Figure 2, which converts xylose into xylulose via xylitol. Xylulose is then converted into xylulose-5-phosphate (X5P) for further metabolism in the pentose phosphate pathway.
E. coli do not exhibit the XR-XDH pathway, instead having an XI pathway that directly converts xylose into xylulose. Hence, together with xylose reductase (BBa_K4324100) which can convert xylose to xylitol, xylitol dehydrogenase presents an alternate xylose metabolism pathway for E. coli.
Furthermore, xylitol dehydrogenase enables E. coli to utilise xylitol as an energy source through its direct conversion to xylulose, which then follows the pentose phosphate pathway.
References
1. https://www.uniprot.org/uniprotkb/Q07993/entry
2. https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-020-1662-x
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