Reporter
mScarlet

Part:BBa_K4321004

Designed by: Kulay Janneh   Group: iGEM22_Guelph   (2022-09-28)


mScarlet

mScarlet is a red fluorescent protein (RFP) which was engineered in 2017 and based on a synthetic gene template consisting of mCherry, chromo proteins, and naturally occurring RFPs.


Usage and Biology

This fluorescent protein carries the highest brightness in the RFP class and a fluorescent life of 3.9 nanoseconds. mScarlet has an absorbance of 569 nanometers and emission maxima of 594 nanometers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 512
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Bindels, D., Haarbosch, L., van Weeren, L. et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods 14, 53–56 (2017). https://doi.org/10.1038/nmeth.4074

mScarlet . SnapGene. (n.d.). Retrieved October 4, 2022, from https://www.snapgene.com/resources/plasmid-files/?set=fluorescent_protein_genes_and_plasmids&plasmid=mScarlet


[edit]
Categories
//cds/reporter/rfp
//classic/reporter
Parameters
color